BACKGROUND Robotic surgery has been widely used in pylorus-preserving gastrectomy (PPG). Segmental gastrectomy (SG) is a classic surgical method in PPG. Indocyanine green (ICG) can increase the number of lymph nodes dissected. CASE SUMMARY We report ICG tracer-guided SG using a robotic system in a 62-year-old female patient with early gastric cancer. It took 23 minutes for robotic setup, 130 minutes for dissection, and 60 minutes for digestive tract reconstruction. There were no postoperative co