• Multiple laser energies were usted to generate and compare distinct LIPSS properties. • Low-power LIPSS reduced the inflammatory profile and enhanced osteogenesis while inhibiting adipogénesis. • HA incorporation was achieved while preserving surface patterns, but no biological benefit was observed. Despite its widespread use in orthopedic implants, titanium exhibits limited bioactivity, which hinders effective osseointegration. To enhance its biological performance, laser-induced periodic sur