Pseudoperonospora cannabina, the causal agent of downy mildew in Cannabis sativa, was first identified in New York in 2020. The pathogen causes angular brown lesions on foliar tissue and dark sporulation on the leaf underside, often leading to leaf curling, defoliation, and yield loss. To date, no sources of genetic resistance or effective chemical controls have been reported. In this study, 108 hemp entries were evaluated for resistance to downy mildew using three phenotyping methods: 1) high-t