IntroductionMicrobial necromass carbon (MNC) is a cornerstone of stable soil organic carbon (SOC), yet how biochar amendments impact its dynamics over time remains poorly understood.MethodsHere, we investigated the temporal response of MNC in a sorghum-cultivated soil amended with vinasse biochar produced at four pyrolysis temperatures (350–650 °C) over a 120-day experiment using amino sugar biomarkers and microbial profiling.ResultsWe reveal a distinct time-dependent biphasic response. Initiall