Frontiers in Microbiology | New and Recent Articles

BackgroundObesity drives chronic diseases such as cardiovascular disease and diabetes. Danlou tablet (DLT), a traditional Chinese medicine formula, is used to treat coronary heart disease by regulating lipid metabolism, suggesting potential for addressing obesity-related metabolic dysfunction. However, its role in obesity and insulin resistance remains unexplored.ObjectivesWe investigated the eff…

Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), poses a severe threat to human health due to the limited efficacy of traditional antibiotics and the rapid emergence of drug resistance. Quaternary ammonium compounds (QACs) are promising antibacterial agents with a membrane-disruptive mechanism that is less prone to inducing resistance. Herein, a series of novel phthalazin…

Dental caries is a chronic biofilm-mediated disease that is caused by the interplay of plaque dysbiosis, persistent acid generation by cariogenic bacteria and host-associated environmental influences. Accordingly, current anti-caries measures increasingly focus on broader interventions targeting bacterial adhesion and colonization, biofilm formation, extracellular polysaccharide (EPS) production,…

The World Health Organization (WHO) has designated carbapenemase-producing Klebsiella pneumoniae (CP-KP) as a critical priority pathogen due to its increasing importance for human health. Although wastewater-based surveillance (WBS) is discussed as a complementary tool for classical systems regarding hazard forecasting and early-warning, methods targeting a single AMR target remain scarce. Theref…

Antimicrobials are widely used in intensive poultry production, particularly in sub-Saharan Africa, promoting the selection and spread of antimicrobial-resistant bacteria. Avian fecal Escherichia coli (AFEC) act as important reservoirs of antimicrobial resistance (AMR) genes, reflecting baseline resistance levels and facilitating dissemination of resistance and virulence determinants within poult…

Methanol is an ideal feedstock for biomanufacturing and can be produced in large quantities from carbon dioxide via photocatalysis or electrolytic reduction, contributing to global carbon neutrality. In this study, we described an engineered Pichia pastoris strain capable of efficiently producing the monoterpene 1,8-cineole from methanol. First, a remarkable high-level synthesis of 1,8-cineole wa…

Microplastic (MP) accumulation in constructed wetlands potentially threatens sulfur-autotrophic denitrification under low C/N conditions, yet the resilience of plastisphere-derived denitrifiers remains poorly understood. Here, we isolated and compared two denitrifying strains, a plastisphere isolate, Stutzerimonas balearica SP-H, and a conventional wetland isolate, Castellaniella denitrificans S0…

Enterovirus A71 (EVA71) is a major pathogen responsible for hand, foot, and mouth disease (HFMD) outbreaks and poses a considerable threat to public health. In this work, we established a molecular detection system that integrates reverse transcription multiple cross displacement amplification (RT-MCDA) with CRISPR/Cas12a technology, targeting the VP1 gene region for EVA71 identification. This pl…

IntroductionThe application of bacteriophages in food systems requires evaluation under conditions that reflect realistic storage scenarios. This study evaluated two lytic bacteriophages, SW03 and CF03, targeting Salmonella enterica and Escherichia coli O157:H7, respectively, as potential biocontrol agents in food matrices.MethodsPhage SW03, isolated from municipal sewage, and phage CF03, isolate…

Nitrogen loss during straw composting substantially undermines nutrient recycling efficiency and the agronomic value of finished compost products. Clarifying how different nitrogen sources mediate nitrogen transformation and nitrogen retention in organic fractions is therefore essential for improving compost maturity and nitrogen retention. This study evaluated how three nitrogen-rich amendments …

Gram-negative bacteria exhibit higher intrinsic resistance to antimicrobial agents than Gram-positive bacteria, largely attributable to their efficient multidrug efflux pumps. Among these, tripartite pumps spanning the inner membrane, periplasm, and outer membrane are central to both intrinsic and acquired resistance. Periplasmic adaptor proteins (PAPs) serve as the core connectors of these syste…

Overexpression of efflux pumps, is a primary mechanism of multidrug resistance in Staphylococcus aureus. This study evaluated grape pomace extracts and their constituent flavonoids as potential efflux pump inhibitors (EPIs) to restore ciprofloxacin susceptibility. RP-HPLC analysis of the extracts identified quercetin, (+)-catechin, and (−)-epicatechin. The synergistic interaction between these ag…

IntroductionHuman herpesvirus 8 (HHV-8) is the etiologic agent of Kaposi sarcoma (KS) and primary effusion lymphoma (PEL), important causes of morbidity and mortality among people living with HIV (PLWH). The highly polymorphic ORF-K1 region is commonly used for HHV-8 genotyping and phylogenetic analysis. We investigated the distribution of HHV-8 genotypes and their clinical correlates among PLWH …

Liver transplantation (LT) is a critical intervention for end-stage liver disease, while complications, such as infections, graft rejection, and metabolic disturbances are common post-transplant. The gut microbiota-bile acid (GM-BA) axis plays a pivotal role in regulating liver function and overall health, influencing both the gut microbiota and bile acid metabolism. This review explored the comp…

Background and aimsFecal microbiota transplantation (FMT) has emerged as a promising therapeutic approach for ulcerative colitis (UC). This single-center retrospective cohort study evaluated the clinical effectiveness and safety of an optimized FMT protocol, in which donor bacteria were concentrated by tangential-flow micropore membrane filtration and delivered after pre-FMT antibiotic preconditi…

Tungsten-containing oxidoreductases (WORs) are a diverse family of enzymes with over 4,000 known members that can be subdivided into 92 clades based on the phylogeny of the large pyranopterin cofactor-containing large subunit (WorL). Despite being widespread in Bacteria and Archaea, particularly in members of the human microbiome, only five of the 92 WOR clades contain a WOR with a defined physio…

Osteoarthritis (OA) has traditionally been viewed as a degenerative joint disorder primarily associated with mechanical stress and progressive structural damage. Increasing evidence, however, indicates that immune imbalance and persistent low-grade inflammation are critically involved in disease initiation and progression. In this context, alterations in the gut microbiota have attracted growing …

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a serious global health threat associated with both nosocomial and community-acquired infections. The reduced efficacy of meropenem due to diverse resistance mechanisms emphasizes the need to investigate genetic determinants and their association with meropenem susceptibility, which is explored in this study. We have performed pan-genome analys…