Salmonella remains a major pathogen in poultry production, posing significant threats to animal health and food safety. In this study, two representative isolates, Salmonella enterica serovar Enteritidis (SA03) and serovar Pullorum (SA406), were selected from multiple isolates recovered from a diseased poultry farm, as predominant serovars identified in the same production environment, for further characterization. Phenotypic identification, antimicrobial susceptibility testing, whole-genome seq