Artificial cerebrospinal fluid preserves neuronal viability and attenuates apoptosis and oxidative stress in HT22 cells

IntroductionArtificial cerebrospinal fluid (ACSF) is critical for maintaining neuronal function during surgical procedures, yet its specific neuroprotective advantages over commonly used irrigation solutions like physiological saline and phosphate-buffered saline (PBS) remain unclear.MethodsThis study compared the neuronal preservation properties of ACSF, saline, and PBS using HT22 mouse hippocampal neurons, with DMEM as a control. Cell viability and apoptosis were assessed using CCK-8 assays an