Salmonella enterica serovar Infantis has emerged over the past two decades as the dominant Salmonella serovar in European Union (EU) broiler production. Due to largely asymptomatic colonization in poultry, multidrug-resistant (MDR) strains can disseminate silently within flocks, leading to contamination of meat products and raising significant One Health concerns. In Italy, S. infantis represents up to 90% of Salmonella isolates from broilers chickens and approximately 40% of human cases, highli